《植物生理学报》 2013, 49(1): 57-62

沙芥叶片活性氧和抗坏血酸对干旱胁迫的响应

庞杰, 郝丽珍*, 张凤兰, 赵鹏, 杨忠仁

内蒙古农业大学农学院, 内蒙古自治区野生特有蔬菜种质资源与种质创新重点实验室, 呼和浩特010019

通信作者：郝丽珍；E-mail: Haolizhen_1960@163.com；Tel: 0471-4318467

摘　要：

本文以沙生蔬菜——沙芥为材料, 研究干旱胁迫下其叶片内活性氧和抗坏血酸的积累规律和相互关系, 为沙生植物干旱胁迫下叶片内活性氧积累和利用提供理论依据, 也为植物干旱胁迫下抗坏血酸调控机制研究提供新的思路。结果表明, 随着土壤相对含水量的下降, 沙芥叶片内AsA、DHA、总AsA、HO·、Pn、Gs含量均呈下降的趋势, 而O2 ¯· 、H2O2和MDA含量呈上升趋势; 同时总AsA与HO·含量极显著正相关, 与H2O2显著正相关, 与O2 ¯· 显著负相关, DHA与HO·显著相关。

关键词：干旱胁迫; 沙芥; 活性氧; 抗坏血酸

收稿：2012-11-02   修定：2012-12-20

资助：公益性行业(农业)科研专项(201203004)、国家自然科学基金(31160393、30860174和30260067)、内蒙古科技攻关项目(20050305和20060202)和内蒙古自然科学基金(2011BS0305和20080404MS0305)。

The Response of Active Oxygen Species and Ascorbic Acid in Pugionium cor-nutum (L.) Gaertn. Leaves to Drought Stress

PANG Jie, HAO Li-Zhen*, ZHANG Feng-Lan, ZHAO Peng, YANG Zhong-Ren

College of Agronomy, Inner Mongolia Agricultural University, Inner Mongolia Autonomous Region Key Laboratory of Wild Pecu-liar Vegetable Germplasm Resource and Germplasm Enhancement, Huhhot 010019, China

Corresponding author: HAO Li-Zhen; E-mail: Haolizhen_1960@163.com; Tel: 0471-4318467

Abstract:

The dynamic change of active oxygen species (ROS) and AsA content in Pugionium cornutum (L.) Gaertn. leaves and possible correlation between them under drought stress were analyzed, which would provide basis for understanding the accumulation and utilization of ROS and new idea for the regulation mechanism of ascorbic acid of psammophytes under drought. The results showed that the content of AsA, DHA, total AsA, HO·, Pn and Gs of Pugionium cornutum (L.) Gaertn. decreased, while the content of O2¯·, H2O2 and MDA in-creased, along with the decrease of the soil relative water content. The correlation between ascorbic acid and several active oxygen species, such as HO·, H2O2 and O2¯· were very significantly positive, significantly positive and significantly negative, respectively, and significant correlation between HO· and DHA were also observed.

Key words: drought stress; Pugionium cornutum (L.) Gaertn.; ROS; AsA